![]() After 9 weeks, the harvest of the bioreactor tissue takes approximately 1 h. ![]() The surgical procedure requires ∼1.5 h to implant four bioreactors adjacent to rib periosteum. The bioreactor packing material can be altered, allowing for the study of various commercially available or novel graft materials. The protocol results in bioreactors that can be harvested for investigation of the effects of different biomaterials for the generation of bone or to generate tissues appropriate for repair of bony defects this protocol focuses on reconstruction of the mandible but could be modified for orthopedic applications. The proposed versatile ovine model can be utilized to investigate the use of a customized bioreactor to generate mineralized tissue, matching the size and shape of a defect before transfer to and integration within another site. The generation of vascularized mineralized tissues of complex geometry without the use of extrinsic growth factors or exogenous cells requires a large animal model to recapitulate the challenges seen in the clinic.
0 Comments
Leave a Reply. |